From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(2R)BSC308
Date: 	Tue, 08 May 2007  08:46:40  -0400  ( 13:46  BST)
Isolation and characterization of Df(2R)BSC308
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC308 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}Vha14[f03593] and P{XP}d11087. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}Vha14[f03593]/P{XP}d11087 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC308 from the segment 
of PBac{WH}Vha14[f03593] to the left of its FRT site and the segment 
of P{XP}d11087 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
Exelixis, Inc. determined the insertion site of P{XP}d11087 to be at 
Release 3 genomic coordinate 11095004 on chromosome arm 2R. This 
corresponds to 52D15 on the Release 5 genome map. The predicted 
position of PBac{WH}Vha14[f03593] on the Release 5 map is 52B5. 
Consequently, the cytological breakpoints of Df(2R)BSC308 are 
predicted to be 52B5;52D15. It failed to complement sli[2].