Date: Tue, 05 Dec 2006 14:55:16 -0500 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(2L)BSC228 Cc: Jill Gresens <jgresensXXXX>, Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufmanXXXX Isolation and characterization of Df(2L)BSC228 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC228 was isolated as a FLP recombinase-induced recombination event involving P{XP}Bsg[d03746] and PBac{WH}Pvr[f06259]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}Bsg[d03746]/PBac{WH}Pvr[f06259] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC228 from the segment of P{XP}Bsg[d03746] to the left of its FRT site and the segment of PBac{WH}Pvr[f06259] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC228 predicted from the Release 4 genomic coordinates of the transposable element insertions sites are 28E5;28F4. It failed to complement Df(2L)BSC111 and Df(2L)Exel7034. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX