From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2L)BSC340
Date: 	Mon, 29 Oct 2007  12:07:18  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2L)BSC340
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC340 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}CG31729[d01914] and PBac{RB}CG31855[e03029]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
P{XP}CG31729[d01914]/PBac{RB}CG31855[e03029] males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC340 from the segment of 
P{XP}CG31729[d01914] to the left of its FRT site and the segment of 
PBac{RB}CG31855[e03029] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. with the substitution of the primer 
5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer 
in the Hybrid PCR protocol in the Supplementary Methods. The 
cytological breakpoints of Df(2L)BSC340 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
34B4;34B8. It failed to complement Nnp-1[k07826] and Df(2L)BSC30.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX