From: Kevin Cook <kcook@XXXX> To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX> Subject: Isolation and characterization of Df(2L)BSC341 Date: Mon, 29 Oct 2007 12:07:18 -0400 ( 16:07 GMT) Isolation and characterization of Df(2L)BSC341 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC341 was isolated as a FLP recombinase-induced recombination event involving P{XP}Catsup[d07915] and PBac{RB}CG17350[e03687]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}Catsup[d07915]/PBac{RB}CG17350[e03687] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC341 from the segment of P{XP}Catsup[d07915] to the left of its FRT site and the segment of PBac{RB}CG17350[e03687] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(2L)BSC341 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 37B11;37D3. It failed to complement Catsup[1] and l(2)37Bb[2]. Df(2L)FDD-0094072 is a synonym for Df(2L)BSC341. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX