From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2R)BSC347
Date: 	Mon, 29 Oct 2007  12:07:18  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2R)BSC347
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC347 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG10936[f02448] and P{XP}d02678. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}CG10936[f02448]/P{XP}d02678 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC347 from the segment 
of PBac{WH}CG10936[f02448]  to the left of its FRT site and the 
segment of P{XP}d02678 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC347 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 54D2;54E9. It failed to complement sub[1] and Df(2R)Exel7149.