From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Subject: 	UAS-Hsap\PFN1 constructs and insertions
Date: 	Fri, 08 Feb 2008  09:42:08  -0500  ( 14:42  GMT)
The following information accompanied stocks donated to the 
Bloomington Stock Center by Jieya Shao and Marc Diamond, University 
of California at San Francisco.
P{UAS-Hsap\PFN1.D} is a P{UAST} construct of Human Profilin 1 (PFN1).
P{UAS-Hsap\PFN1.D}2 is a homozygous viable and fertile, second 
chromosome insertion.
P{UAS-Hsap\PFN1.S137A} is a similar construct encoding a mutant PFN1 
protein with a S137A amino acid substitution.  Likewise, 
P{UAS-Hsap\PFN1.S137D} encodes PFN1 with a S137D substitution.
P{UAS-Hsap\PFN1.S137A}2 is a homozygous viable and fertile, second 
chromosome insertion.
P{UAS-Hsap\PFN1.S137A}3 and P{UAS-Hsap\PFN1.S137D}3 are homozygous 
viable and fertile, third chromosome insertions.
Neither the S137A nor S137D substitutions affect the binding of PFN1 
to actin as assayed by immunoprecipitation.  The S137D protein 
variant does not bind huntingtin, whereas the S137A variant does.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX