From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC443
Date: 	Tue, 15 Apr 2008  14:38:31  -0400  ( 19:38  BST)
Isolation and characterization of Df(3L)BSC443
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC443 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f01147 and P{XP}roq[d08799]. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}f01147/P{XP}roq[d08799] males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC443 from the segment of 
PBac{WH}f01147 to the left of its FRT site and the segment of 
P{XP}roq[d08799] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3L)BSC443 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 72B1;72E4. Df(3L)BSC443 failed to complement brm[2], Mbs[3] 
and Taf4[1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX