From: Kevin Cook <kcook@XXXX> To: flybase-updates@XXXX Cc: Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX> Subject: Isolation and characterization of Df(2L)BSC407 Date: Tue, 15 Apr 2008 14:14:22 -0400 ( 19:14 BST) Isolation and characterization of Df(2L)BSC407 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC407 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}CG6579[e01628] and P{XP}pdm2[d09994]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBac{RB}CG6579[e01628]/P{XP}pdm2[d09994] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC407 from the segment of PBac{RB}CG6579[e01628] to the left of its FRT site and the segment of P{XP}pdm2[d09994] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(2L)BSC407 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 33D2;33F2. It failed to complement bun[00255], Elf[LR16] and aret[01284]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX
