From: Kevin Cook <kcook@XXXX> To: flybase-updates@XXXX Subject: Isolation and characterization of Df(3R)BSC492 Date: Tue, 15 Apr 2008 15:10:18 -0400 ( 20:10 BST) Isolation and characterization of Df(3R)BSC492 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC492 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG13606[f00488] and P{XP}bai[d09741]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBac{WH}CG13606[f00488]/P{XP}bai[d09741] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC492 from the segment of PBac{WH}CG13606[f00488] to the left of its FRT site and the segment of P{XP}bai[d09741] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC492 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 95E7;96B17. Df(3R)BSC492 failed to complement jar[322], ash2[1] and tld[B4].