From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Subject: 	Isolation and characterization of Df(3R)BSC498
Date: 	Tue, 15 Apr 2008  15:14:13  -0400  ( 20:14  BST)
Isolation and characterization of Df(3R)BSC498
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC498 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f01132 and P{XP}d05341. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}f01132/P{XP}d05341 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC498 from the segment of 
PBac{WH}f01132 to the left of its FRT site and the segment of 
P{XP}d05341 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(3R)BSC498 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
98A4;98B5. Df(3R)BSC498 failed to complement raps[193] and Df(3R)BSC42.