From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Subject: 	Isolation and characterization of Df(2R)BSC482
Date: 	Tue, 15 Apr 2008  15:04:29  -0400  ( 20:04  BST)
Isolation and characterization of Df(2R)BSC482
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC482 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}l(2)k05713[d11115] and 
PBac{WH}Strn-Mlck[f04333]. The deletion was isolated as a chromosome 
lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; 
P{XP}l(2)k05713[d11115]/PBac{WH}Strn-Mlck[f04333] males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC482 from the segment of 
P{XP}l(2)k05713[d11115] to the left of its FRT site and the segment 
of PBac{WH}Strn-Mlck[f04333] to the right of its FRT site. Its 
presence was verified using the PCR methods and primers described in 
Parks et al. The cytological breakpoints of Df(2R)BSC482 predicted 
from the Release 5 genomic coordinates of the transposable element 
insertions sites are 52C8;52D5. Df(2R)BSC482 failed to complement 
Df(2R)BSC308 and Df(2R)Exel7138.