From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2R)BSC427
Date: 	Tue, 15 Apr 2008  14:27:21  -0400  ( 19:27  BST)
Isolation and characterization of Df(2R)BSC427
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC427 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}e04001 and P{XP}d00680. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{RB}e04001/P{XP}d00680 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC427 from the segment of 
PBac{RB}e04001 to the left of its FRT site and the segment of 
P{XP}d00680 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. with the 
substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the 
RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the 
Supplementary Methods. The cytological breakpoints of Df(2R)BSC427 
predicted from the Release 5 genomic coordinates of the transposable 
element insertions sites are 52A10;52D2. It failed to complement 
Df(2R)BSC308 and Df(2R)Exel6285.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX