From: Kevin Cook <kcook@XXXX> To: flybase-updates@XXXX Cc: Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>, kaufman@XXXX Subject: Isolation and characterization of Df(1)BSC532 Date: Wed, 11 Jun 2008 14:30:00 -0400 ( 19:30 BST) Isolation and characterization of Df(1)BSC532 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC532 was isolated as a FLP recombinase-induced recombination event involving P{XP}norpA[d07261] and PBac{WH}f07985. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}norpA[d07261]/PBac{WH}f07985; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC532 from the segment of P{XP}norpA[d07261] to the left of its FRT site and the segment of PBac{WH}f07985 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f07985 to be Release 3 genomic coordinate 4549729 on the X chromosome. This corresponds to 4D2 on the Release 3 and Release 5 genome maps. The predicted position of P{XP}norpA[d07261] on the Release 5 map is 4C1. Consequently, the cytological breakpoints of Df(1)BSC532 are predicted to be 4C1;4D2. It failed to complement rb[1], bi[1] and peb[1]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX
