From: lcherbas@XXXX
Subject: FlyBase personal communication:  miscellaneous transformed cell lines
Date: October 16, 2008  11:37:28  AM GMT- 04:00 
Origin of miscellaneous transformed cell lines
Lucy Cherbas
Drosophila Genomics Resource Center, Indiana University
S2-MtN (FBtc0000154) is a stably transformed clonal line made from S2 (FBtc000006) by transformation with a mixture of p8HCO (FBrf0053391), a plasmid expressing methotrexate resistance, and pMtNMg (FBrf0053391), a plasmid expressing full-length Notch from a metallothionein promoter. The cell line was made as part of a collaboration between the Cherbas lab, the Muskavitch lab, and the Artavanis-Tsakonis laboratory.
S2-Tub-wg (FBtc0000165) is a stably transformed population of Schneider's line 2 cells (FBtc0000999) containing a plasmid in which wg protein is expressed from a tubulin promoter; the line constitutively exports WG into the medium. The line was made and donated to the DGRC by Roel Nusse's lab; the information given here was obtained from Roel Nusse. I do not have details about the structure of the expression plasmid or the resistance marker.
S2-Mt-GFP-alphaTub84B (FBtc0000168) is a stably transformed population of Schneider's line 2 cells (FBtc0000999) containing a plasmid in which a tubulin84B-GFP fusion protein is expressed from a copper-inducible metallothionein promoter. The cells carry a hygromycin-resistance marker. The cells were contributed to the DGRC by S. L. Rogers, along with the above description. I do not know details of the expression plasmid or the resistance marker.
Lucy
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Lucy Cherbas
Department of Biology and
Drosophila Genomics Resource Center
Jordan Hall
1001 East Third Street
Indiana University
Bloomington, IN 47405