From: kcook@XXXX
Subject: Isolation and characterization of Df(2R)BSC695
Date: December 28, 2008 1:18:58 PM GMT- 05:00
Isolation and characterization of Df(2R)BSC695
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC695 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG30184f04532 and P{XP}d11424. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG30184f04532/P{XP}d11424 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC695 from the segment of PBac{WH}CG30184f04532 to the left of its FRT site and the segment of P{XP}d11424 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d11424 to be Release 3 genomic coordinate 19035686 on chromosome arm 2R. The Gene Disruption Project determined the insertion site of P{XP}d11424 to be Release 3 coordinate 19035692 on chromosome arm 2R. This corresponds to 60B1 on the Release 5 genome map. The predicted position of PBac{WH}CG30184f04532 on the Release 5 map is 59F1. Consequently, the cytological breakpoints of Df(3R)BSC695 are predicted to be 59F1;60B1. Df(2R)BSC695 failed to complement retn02535, or49h and bgcn1.
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Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-7005 kcook@XXXX 
