From: kercook@XXXX Subject: Isolation and characterization of Df(3L)BSC774 Date: 6 May 2009 00:39:10 BST To: flybase-cambridgeXXXX, sjchristXXXX, ruacook@XXXX Isolation and characterization of Df(3L)BSC774 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC774 was isolated as a FLP recombinase-induced recombination event involving P{XP}d05671 and PBac{WH}f06011. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d05671/PBac{WH}f06011 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC774 from the segment of P{XP}d05671 to the left of its FRT site and the segment of PBac{WH}f06011 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC774 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3L:15693003 ;16233373..16233380 and the cytological breakpoints predicted from these coordinates are 71F1;72D10. Df(3L)BSC774 failed to complement brm2, Mbs3 and Taf41. -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu
