From: kercook@XXXX Subject: Isolation and characterization of Df(2R)BSC779 Date: 6 May 2009 00:40:20 BST To: flybase-cambridgeXXXX, sjchristXXXX, ruacook@XXXX Isolation and characterization of Df(2R)BSC779 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC779 was isolated as a FLP recombinase-induced recombination event involving P{XP}aptd01747 and PBac{WH}f02049. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}aptd01747/PBac{WH}f02049 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC779 from the segment of P{XP}aptd01747 to the left of its FRT site and the segment of PBac{WH}f02049 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2R)BSC779 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2R:19468319 ;19664298 and the cytological breakpoints predicted from these coordinates are 59F1;59F7. Df(2R)BSC779 failed to complement retn02535. -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu
