Isolation and characterization of Df(2L)BSC892.
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC892 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08229 and PBac{WH}loqsf00791. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; P{XP}d08229/PBac{WH}loqsf00791 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC892 from the segment of P{XP}d08229 to the left of its FRT site and the segment of PBac{WH}loqsf00791 to the right of its FRT site. The breakpoints of Df(2L)BSC892 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2L:13060152 ;13382714 and the cytological breakpoints predicted from these coordinates are 34A5;34B9. Df(2L)BSC892 failed to complement Nnp-1k07826 and Df(2L)BSC340. Heterozygous Df(2L)BSC892 females are sterile.
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Kevin Cook, Ph.D.
Bloomington Drosophila Stock Center
Department of Biology
Indiana University
1001 E. Third St.
Bloomington, IN 47405-7005
