The following information accompanied stocks donated to the Bloomington Stock Center by Peter Smibert, U. Melbourne. Peter's description of the screen: "We screened for new lethal mutations in the region uncovered by Df(3L)ED220 (3L: 16,080,584 - 16,404,777). We performed 7 rounds of EMS mutagenesis (standard protocol - 8 hours starvation followed by 25mM EMS in sucrose, overnight feeding). For rounds 1 and 2, our starting strain was an isogenised w1118 stock. For rounds 3 onwards, we used w ; FRT80B. Mutagenised males were crossed to virgin females carrying TM6B. F1 male progeny carrying TM6B were individually crossed to w ; Df(3L)ED220 / TM6B and these crosses were scored for viability of non-Tb animals. Df(3L)ED220 is marked with w+, so stocks were recovered from vials with no viable non-Tb progeny by selecting white- males and virgin females. All stocks are balanced with TM6B, Hu, Tb. Alleles are named as follows (for example) 5B4, where 5 means round 5 of mutagenesis, B is bottle B and 4 means the 4th putative lethal detected in the initial screening. Alleles with either the first number or letter different are considered to be independent." New alleles of existing complementation groups include: l(3)72De1A7 l(3)72De3A1 l(3)72Df1A1 l(3)72Df4C2 l(3)72Dg1A10 l(3)72Dg7A1 l(3)72Di1A16 l(3)72Di4B5 Taf41A4 Taf47A8 l(3)72Dk1A14 l(3)72Dk5B1 l(3)72Dn3A9 l(3)72Dn5B4 l(3)72Do5A3 l(3)72Dp2A4 l(3)72Fa1A3 l(3)72Fa4A2 Alleles of novel complementation groups include: l(3)ED220-12A3 l(3)ED220-17A10 l(3)ED220-23A2 l(3)ED220-26B2 Phenotypic descriptions: l(3)72De small clear looking 1st/2nd instar larvae crawling out of food l(3)72Df l(3)72Dg l(3)72Di degenerating pupae Taf4 1A4 has some 1st instar larvae, 7A8 gets to pharate adult. l(3)72Dk die as pupae l(3)72Dn l(3)72Do l(3)72Dp l(3)72Fa early larval lethal l(3)ED220-1 degenerating pupae l(3)ED220-2 early larval lethal -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center http://flystocks.bio.indiana.edu
