Basal X chromosome segments present on Dp(1;Y)BSC chromosomes Kim Cook, Russell Garton, Adam Brown, Megan Ward, Jennifer Deal, Megan Deal, Thom Kaufman & Kevin Cook Bloomington Drosophila Stock Center Indiana University We have generated Y-linked duplications of X chromosomal segments by irradiating an attached-XY (C(1;Y)N12) with an inversion in the X chromosome. Irradiation produces a Dp(1;Y) chromosome by inducing a large deletion in the progenitor chromosome with one breakpoint within the inversion and another breakpoint in X heterochromatin or basal X euchromatin. The region distal to the deletion on a Dp(1;Y) consists of two parts: X tip genes distal to the inversion and genes from the middle of the X moved near the end of the X by the inversion. The distal and medial duplicated segments present on particular Dp(1;Y)BSC chromosomes have been described in other personal communications to FlyBase. The region of a Dp(1;Y) proximal to the deletion will carry no basal euchromatic genes if the proximal deletion breakpoint falls in X heterochromatin. When the proximal deletion breakpoint falls in basal X euchromatin, however, basal euchromatic genes will be present. This personal communication describes the basal X segments present on Dp(1;Y)s derived from In(1)BSC16 and In(1)BSC27. We mapped the distal extents of the basal segments to ~10 gene intervals defined by PCR primers using the following approach. Males carrying a Dp(1;Y) were crossed to females carrying a transposable element insertion in a basal X position. Primers flanking the insertion sites of the transposable elements were used to amplify fragments from DNA isolated from male progeny. With short extension times, fragments are amplified only if the site of the transposable element insertion is present on the Dp(1;Y). The following transposable elements and primer pairs were used in mapping: Insertion: P{EPgy2}EY09781 Forward primer: CGCACAGGTCTTGAAGGCATTGCC ( X:21443126..21443149 ) Reverse primer: GTTTCTAGCAGCCCCCTTCGCACCG ( X:21443688..21443712 ) Insertion: P{Mae-UAS.6.11}CG14619GG01842 Forward primer: CATCGCTCGCGCGCACAATCTCGGC ( X:21858117..21858141 ) Reverse primer: GTGCCGCCAGGCTGTCTACGCTCC ( X:21858613..21858636 ) Insertion: P{GT1}BG01274 Forward primer: CTAGTGTATTTGCCTTCACCATAATCG ( X:21961730..21961756 ) Reverse primer: GAGCGAGGAATATCTGATATGCGG ( X:21962214..21962237 ) Insertion: PBac{WH}f02323 Forward primer: GATGGCGGTGTCCTTGTCTCTGGC ( X:22366703..22366726 ) Reverse primer: CCTGTGACATTAATGCAGGCGACGG ( X:22367213..22367237 ) The following basal segment is present on a Dp(1;Y) chromosome (Release 5 coordinates and predicted cytologies). Dp(1;Y)BSC226 carries basal euchromatic genes: Dp(1;Y)BSC226 X:21443126--21858117 ;h28--h29 (20A3-20C1;h28--h29) PBac{WH}f02323, an insertion immediately distal to stnA in 20F3, was the most basal transposon insertion site we tested by PCR. The following Dp(1;Y)s do not carry this insertion site and, consequently, do not duplicate basal euchromatic genes distal to stnA. Dp(1;Y)BSC266 Dp(1;Y)BSC223 Dp(1;Y)BSC224 Dp(1;Y)BSC225 Dp(1;Y)BSC227 Dp(1;Y)BSC267 Dp(1;Y)BSC231 Dp(1;Y)BSC268 Dp(1;Y)BSC269 Dp(1;Y)BSC270 Dp(1;Y)BSC271 Dp(1;Y)BSC272 Dp(1;Y)BSC273 Dp(1;Y)BSC274 Dp(1;Y)BSC275