For each of the following lines, could you provide the reference in which the line was generated, identify the line in FlyBase, or, if the line is newly generated in your lab, provide some molecular details as to how the line was generated. 1. UAS-hpo 2. Hsp70pb-GAL4 3. UAS-droncRNAi 4. dronc1.7kb-lacZ 5. dronc2.8kb-lacZ Response to Flybase queries: Verghese et al., 2012 1) Hsp70pb-GAL4: Was obtained from Bloomington (P{GAL4-Hsp70.PB} 2, FlyBase ID: FBti0001001 Stock # BL2077) 2) UAS hpo: two different UAS Hpo lines were used for the experiments described in Verghese et al., 2012. These lines were generated by Madhuri Kango-Singh in the Halder lab and published in the reference below. - GMR GAL4 UAShpo: UAS hpo (full length ORF) - nubGAL4 UAShpo: UAS hpo INH (Δauto) Reference: Hippo promotes proliferation arrest and apoptosis in the Salvador/Warts pathway. Udan RS, Kango-Singh M, Nolo R, Tao C, Halder G. Nat Cell Biol. 2003 Oct; 5(10): 914-20. 3) UASdroncRNAi: Stock # NIG-Fly 8091 R-2 III, was obtained from the Genetic Strain Research Center, National Institute of Genetics Mishima, Shizuoka, 411-8540, Japan. 4) Dronc 2.8kb-lacZ, and Dronc 1.7kb-lacZ: These lines were obtained from Dr. A. Bergmann, and were originally generated in the Lab of Dr. Sharad Kumar, Molecular Regulation Laboratory, Centre for Cancer Biology, Adelaide, Australia. Kindly note that the dronc1.7kb-lacZ was made by the Kumar lab and distributed to the fly community but not published in the paper below that describes other dronc-lacZ lines including dronc2.8kb-lacZ Reference: Distinct promoter regions regulate spatial and temporal expression of the Drosophila Caspase dronc. TJ Daish, D Cakouros and Sharad Kumar. Cell Death and Differentiation (2003) 10, 1348-1356.