Yu, Z., Ren, M., Wang, Z., Zhang, B., Rong, Y.S., Jiao, R., Gao, G. (2013). Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila. Genetics 195(1): 289--291.
FlyBase ID
FBrf0222617
Publication Type
Research paper
Abstract
We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.
