The Heidelberg CRISPR Fly Design Library (HD_CFD) collection consists of fly stocks containing transgenic constructs designed for conditional CRISPR/Cas9 mutagenesis of a gene of interest; a GAL4 driver can be used to express a HD_CFD construct and a UAS-Cas9 transgene in a tissue-specific manner, inducing mutagenesis only in the GAL4-expressing cells.
In each HD_CFD construct, UAS regulatory sequences drive expression of two different sgRNAs, both of which target common coding exons within the 5' half of a single gene of interest without overlapping the start codon; this is designed to result in an indel (small insertion or deletion mutation) of the target gene in the presence of a source of Cas9.
The 'Associated Files' for this personal communication contain details of the HD_CFD fly stocks available at the Vienna Drosophila Resource Center. The file contains data including the vector used to generate the construct, the sgRNA sequences present and the gene targeted by the sgRNAs.