Shekhar, S., Moehlman, A.T., Park, B., Ewnetu, M., Tracy, C., Titos, I., Pawłowski, K., Tagliabracci, V.S., Krämer, H. (2023). Allnighter pseudokinase-mediated feedback links proteostasis and sleep in Drosophila. Nat. Commun. 14(1): 2932.
FlyBase ID
FBrf0256541
Publication Type
Research paper
Abstract
In nervous systems, retrograde signals are key for organizing circuit activity and maintaining neuronal homeostasis. We identify the conserved Allnighter (Aln) pseudokinase as a cell non-autonomous regulator of proteostasis responses necessary for normal sleep and structural plasticity of Drosophila photoreceptors. In aln mutants exposed to extended ambient light, proteostasis is dysregulated and photoreceptors develop striking, but reversible, dysmorphology. The aln gene is widely expressed in different neurons, but not photoreceptors. However, secreted Aln protein is retrogradely endocytosed by photoreceptors. Inhibition of photoreceptor synaptic release reduces Aln levels in lamina neurons, consistent with secreted Aln acting in a feedback loop. In addition, aln mutants exhibit reduced night time sleep, providing a molecular link between dysregulated proteostasis and sleep, two characteristics of ageing and neurodegenerative diseases.
