FB2024_03 , released June 25, 2024
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Smith, D. (2023.9.14). Location data for Ance-3 mutations. 
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FBrf0257614
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Personal communication to FlyBase
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The intronic sequence around Ance-31 are:
gtttcgtttttgtttatctctggtctgcacg
the last c is mutated to an a (acc—>aag)
For Ance-33
the sequence immediately upstream of the deletion is
GTGGCATTTTTACCTTTTGCATTATCTCTG
The sequence just past the deletion is
GCTGCATAACCAGAGCAATTAAAGCGTCTAGCAGC
For Ance-3KO (reported as ance-3RFP),
the sequence of the CRISPR upstream is CAACGGCGACCTTGGCAGTGTGG-the PAM site is underlined-this is 30 bp upstream of the ATG.
The downstream CRISPR site is GCAATTAAAGCGTCTTAGCAGCGG.
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