FB2024_03 , released June 25, 2024
Sequence Feature: Dmel\dsRNA-GD5375
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General Information
Symbol
Dmel\dsRNA-GD5375
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000075098
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:12,339,033..12,339,362 [+]

Genomic Maps
Sequence Data
Length
330
Comments

One copy of repeat shown

(Dietzl et al., 2007)

Sequence

Sequence Downloader
GACTTCAACAAGCTGCCGATCTTCCGCTTTTTCAACTGGACGAAAAAATAATGGCATATAATGAGCTCAATCTTATCCAG
CGGACTGACATCTCCTCGGTTTCAACTAGCGAGCCAGTGATCCGGAATGGTAGCTCTTCCGGGGATGGACCAGCATTGCG
GCAGGGTATGACATACACCCAGTTCGAAAGGCAAATCCTTAGACTCCTTAGGAGGCTTGGCATTTACGATAGCTTCACTG
TTCGCGTATTCAAGGCAATCTTCAGCGATGAAGAGCAGCTAAAAAAGCTCAAAAAGAAGCTGGACGAGTTGGGAAAGAAG
GATAAGGACA
Associated Information
Gene(s) (targeted or local)
Allele(s)
Transcripts(s)
    Polypeptide(s)
      Construct
      Experimental Data
      Progenitors
      PCR template

      genomic DNA

      (Dietzl et al., 2007)
      Primer 1 
      GACTTCAACAAGCTGCCGATCTTCC
      (Dietzl et al., 2007)
      Primer 2 
      TGTCCTTATCCTTCTTTCCCAACTCGTC
      (Dietzl et al., 2007)
      Comments
      Collection Information
      Collection: VDRC-GD
      Symbol
      VDRC-GD
      Title
      A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC first generation.
      Source and Progenitors
      Species of derivation
      D. melanogaster
      Strain of derivation
      Vector of progenitor construct
      pP{MF3}
      Description

      Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

      (Dietzl et al., 2007)

      NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

      (FlyBase Curators, 2013)
      Additional data
      Sample preparation
      Collection preparation

      A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.

      (Dietzl et al., 2007)
      Mode of assay
      Data analysis

      A specificity score (Q) was calculated for each construct, such that if Q is < 0.5, off-target effects are probable and if Q > 0.8, off-target effects are improbable.

      (Dickson et al., 2007.7.18)
      Cell lines
      Observed in
      Not observed in
      Comments
      Stocks (1)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      5375
      (Dietzl et al., 2007)
      dsRNA-GD5375
      Secondary FlyBase IDs
      • FBri0072268
      References (1)