FB2024_04 , released June 25, 2024
Cell Line: S2R+
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General Information
Symbol
S2R+
Species
D. melanogaster
Feature type
cultured cell-line
FlyBase ID
FBtc0000150
Source
Oregon R
(Cherbas, 2008.11.12)
Tissue Source
embryo
(Schneider, 1972)
Developmental Stage
late embryonic stage
(Cherbas, 2008.11.12)
Lab of Origin

Schneider

(Schneider, 1972)
Characterization
Sex
male (based on expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl)
(Lee et al., 2014)
Karyotype
Associated Genes (0)
    Integrated Constructs (0)
      Comments

      New stable cell line derived from this cell line: A N-expressing stable S2R+ cell line was created.

      (Shimizu et al., 2024)

      New stable cell line derived from this cell line: Stable S2R+ cell lines expressing 1) AkhR and GFP.rl, 2) AkhR and GFP.SKL, or 3) AkhR and APEX2.rl were generated.

      (Li et al., 2023)

      New stable cell line derived from this cell line: Stable S2R+ cell lines were generated that stably express the metallothionein promoter-driven SAM (synergistic activation mediator) complex.

      (Xia et al., 2023)

      S2 cells were obtained from the laboratory of Norbert Perrimon.

      (Li et al., 2023)

      S2R+ cells were obtained from the DRSC.

      (Xu et al., 2023)

      New stable cell line derived from this cell line: A stable S2 cell line deleted for the Pex1 gene was created.

      (Nath et al., 2022)

      New stable cell line derived from this cell line: A stable cell line overexpressing eGFP-GPAT4 was created. Seipin knock-in and knock-out cell lines were created.

      (Song et al., 2022)

      New stable cell line derived from this cell line: S2R+ cells stably expressing Actin-Gal4 ( source:Prof . Satyajit Mayor lab, NCBS, Bangalore-India).

      (Kumari et al., 2022)

      New stable cell line derived from this cell line: S2R+ cells stably expressing LiveDrop-GFP or f S2 cells stably expressing LiveDrop-RFP were used.

      (Ueda et al., 2022)

      New stable cell line derived from this cell line: Stable S2R+ cell lines were created carrying C-terminally HA-tagged forms of either COX7B or human COX7B.

      (Brischigliaro et al., 2022)

      New stable cell line derived from this cell line: Stable S2R+ cell lines were generated carrying C-terminally HA-tagged forms of either COX7B or hhuman COX7B.

      (Brischigliaro et al., 2022)

      New stable cell line derived from this cell line: Stable cell lines MT-sloth1, MT-sloth2, and MT-sloth1/2 were created.

      (Bosch et al., 2022)

      New stable cell line derived from this cell line: Stable cell lines co-expressing Snap25 (wildtype or P125A mutant) in combination with either Hip14, app, or Patsas.

      (Porcellato et al., 2022)

      New stable cell line derived from this cell line: A Tag:FLAG was inserted in the endogenous cav locus using CRISPR/Cas9.

      (On et al., 2021)

      New stable cell line derived from this cell line: An Act-PE2 (prime editor 2) cell line was generated for use in prime editing. PE2 is expressed under the Act5C promoter.

      (Bosch et al., 2021)

      New stable cell line derived from this cell line: S2R+ cell expressing wildtype or mutant Tbce from the Act5C promoter were created.

      (Métivier et al., 2021)

      New stable cell line derived from this cell line: S2R+ cells were stably transfected with copper-inducible Myc-tagged CkIalpha.

      (Hirschhäuser et al., 2021)

      New stable cell line derived from this cell line: The SR9rg cell line was generated in which GFP reporter transgenes can be assembled efficiently by site-directed chromosomal integration of a test DNA fragment, allowing measurement of its CRE activity by flow cytometry.

      (Bai et al., 2021)

      S2R + cells were obtained from the laboratory of Sven Bogdan.

      (Chartschenko et al., 2021)

      S2R+ (FEx 2.5%), S2R+ cells supplemented with adult fly extract used.

      (Luhur et al., 2021)

      S2R+ cells were obtained from the Carthew lab.

      (Melo et al., 2021)

      S2R+ cells were obtained from the laboratory of N. Perrimon.

      (Ding et al., 2021, Zhao et al., 2021)

      Source of S2R+ cells: DGRC.

      (Kula-Eversole et al., 2021, Singh et al., 2021)

      Source of S2R+ cells: E. Chen (UT Southwestern).

      (Kim et al., 2021)

      New stable cell line derived from this cell line: A stable S2R+ cell line was generated lacking endogenous Stat92E by CRISPR/Cas9-mediated mutatgenesis.

      (Moore et al., 2020)

      New stable cell line derived from this cell line: S2R+ cells in which the fdl gene is deleted were obtained from the laboratory of D. Jarvis.

      (Akintayo et al., 2020)

      New stable cell line derived from this cell line: Stable cell lines containing separase cleavage reporter constructs consisting of the MtnA promoter driving expression of cid for centromere localization fused to RFP(Unk) and EGFP separated by uno or vtd wild-type and mutant separase cleavage sites.

      (Weber et al., 2020)

      New stable cell line derived from this cell line: expressing RNAi resistant versions of RN-tre tagged with Tag:MYC under the control of a MT promoter.

      (Platenkamp et al., 2020)

      Both S2R+ and S2R+Wb (Wolbachia) cells were used.

      (Fattouh et al., 2019)

      New stable cell line derived from this cell line: An S2R+ cell line with a knockout gig mutation was used.

      (Kim and Choi, 2019)

      New stable cell line derived from this cell line: Stable S2R+ cell lines containing C-terminal and N-terminal fusions of Vap33 or Vap33(P58S) with GFP were created.

      (Chaplot et al., 2019)

      S2R+ cells were obtained from the laboratory of N. Yanagawa.

      (Imai et al., 2019)

      New stable cell line derived from this cell line: S2R+ cells stably expressing Actin-GAL4 (obtained from the laboratory of Satyajit Mayor, NCBS, Bangalore) were used.

      (Balakrishnan et al., 2018)

      New stable cell line derived from this cell line: S2R+ cells stably expressing Actin-GAL4 were used.

      (Panda et al., 2018)

      New stable cell line derived from this cell line: S2R+ cells were stably transfected with a pCasper-tub-mitoGFP encoding a tubulin promoter driven human COX VIII mitochondrial targeting signal fused to the N-terminus of EGFP.

      (Anding et al., 2018)

      New stable cell line derived from this cell line: Tag:FLAG-Tag:HA-AGO2 stable S2R+ cells from the laboratory of Eric Lai were used.

      (Lin et al., 2018)

      New stable cell line derived from this cell line: Stable cell lines were generated to express constructs containing destabilizing domains (genetic tags that conditionally control the level of abundance of proteins of interest with specific stabilizing small-molecule drugs).

      (Kogenaru and Isalan, 2018)

      New stable cell line derived from this cell line: a stable S2R+ cell line expressing AkhR was generated.

      (Song et al., 2017)

      New stable cell line derived from this cell line: stably transfected with transferrin receptor.

      (Hemalatha et al., 2016)

      Expression of general hemocyte markers observed.

      (Cherbas et al., 2011)

      Expression profiling by genome tiling array for this cell line may be found at GEO: GSE16287 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16287).

      (Cherbas et al., 2011)

      New stable cell line derived from this cell line: cell line expressed Atg8a.

      (Wilkinson et al., 2009)

      S2R+ is an isolate of S2 cells that was found in the Miyake laboratory freezer. It was contributed to the Miyake lab by Imogene Schneider and is likely to be very similar to the original S2 line.

      (Cherbas, 2008.11.12)

      S2R+ is an isolate of S2 that has receptors for wg signalling. It is more strongly adherent to the substrate than other S2 cells available from the DGRC.

      (Cherbas, 2008.11.12)

      Hemocyte-like gene expression, phagocytic, adherent, flat cells; Fz+ and Wg-responsive.

      (Ramadan et al., 2007)

      S2R+ cells express fz and fz2, and respond to the addition of extracellular wg protein by elevating arm protein levels and hyperphosphorylating dsh protein.

      (Yanagawa et al., 1998)
      Parental Lines (1)
      Isolate of:
      Descendant Lines (18)
      External Stocks and Resources ( 3 )
      Crossreferences
      Cellosaurus - Cellosaurus is a knowledge resource on cell lines that attempts to describe all cell lines used in biomedical research.
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      RRID - Research Resource Identification Portal
      Synonyms and Secondary IDs (20)
      Reported As
      Symbol Synonym
      CVCL_Z831
      (Bairoch, 2016)
      DGRC 150
      (Rosin et al., 2018)
      DGRC S2R+
      (Weaver et al., 2022)
      FBtc0000150
      (Bawankar et al., 2021)
      RRID:CVCL_Z831
      (Bairoch, 2016)
      S2
      (Känel et al., 2022, Arquier et al., 2021, Palacios et al., 2021, Singh et al., 2021, Westholm et al., 2014, Cherbas, 2008.11.12)
      S2 receptor plus
      (Yanagawa et al., 1998)
      S2-R+
      (Schellinger et al., 2022, Joseph et al., 2018, Praktiknjo et al., 2018, Zhou et al., 2018, Yao et al., 2006)
      S2-R +
      (Ueberschär et al., 2019)
      S2R
      (Renschler et al., 2018, Kanoh et al., 2015, Menzel et al., 2008)
      S2R +
      (Chartschenko et al., 2021)
      S2R+
      (Busseau et al., 2024, Kogenaru et al., 2024, Lee et al., 2024, Bar-Cohen et al., 2023, Borkowsky et al., 2023, Carter et al., 2023, Corrà et al., 2023, Drees et al., 2023, Ewen-Campen et al., 2023, Fontana et al., 2023, Fulford et al., 2023, Ghosh et al., 2023, Klucnika et al., 2023, Krämer et al., 2023, Layana et al., 2023, Li et al., 2023, Li et al., 2023, Mattedi et al., 2023, Mauri et al., 2023, Monteiro et al., 2023, Park et al., 2023, Rader et al., 2023, Rothenberg et al., 2023, Shi et al., 2023, Shu et al., 2023, Tang et al., 2023, Terretaz et al., 2023, Ueda et al., 2023, Wippich et al., 2023, Xia et al., 2023, Xu et al., 2023, Zang and Bashaw, 2023, Zappia et al., 2023, Zhao et al., 2023, Borkúti et al., 2022, Bosch et al., 2022, Bose et al., 2022, Brischigliaro et al., 2022, Carnesecchi et al., 2022, Cheng et al., 2022, Dalton et al., 2022, Del Castillo et al., 2022, Ding et al., 2022, Emtenani et al., 2022, Gu et al., 2022, Han et al., 2022, Han et al., 2022, Ho et al., 2022, Horard et al., 2022, Joseph et al., 2022, Kabakci et al., 2022, Kabakci et al., 2022, Känel et al., 2022, Karki et al., 2022, Kumari et al., 2022, Lehne et al., 2022, Lewerentz et al., 2022, Lu et al., 2022, Lusk et al., 2022, Mariyappa et al., 2022, Mlih and Karpac, 2022, Nath et al., 2022, Porcellato et al., 2022, Ribeiro et al., 2022, Shen et al., 2022, Singh and Kim, 2022, Singh et al., 2022, Song et al., 2022, Starke et al., 2022, Tendulkar et al., 2022, Ueda et al., 2022, Villars et al., 2022, Wang et al., 2022, Wu et al., 2022, Xu et al., 2022, Xu et al., 2022, Zang et al., 2022, Øvrebø et al., 2022, Arquier et al., 2021, Bai et al., 2021, Bajusz et al., 2021, Basak et al., 2021, Bawankar et al., 2021, Bejarano et al., 2021, Benincá et al., 2021, Bosch et al., 2021, Brischigliaro et al., 2021, Chaudhari et al., 2021, Ding et al., 2021, Dong et al., 2021, Feng et al., 2021, Formicola et al., 2021, Gore et al., 2021, Hirschhäuser et al., 2021, Joseph and Lai, 2021, Kim et al., 2021, Kula-Eversole et al., 2021, Lee et al., 2021, Lee et al., 2021, Luhur et al., 2021, Maglioni et al., 2021, Mariyappa et al., 2021, Melo et al., 2021, Métivier et al., 2021, On et al., 2021, Rai et al., 2021, Saad and Hipfner, 2021, Safieddine et al., 2021, Serratì et al., 2021, Srivastava et al., 2021, Stephenson et al., 2021, Tamada et al., 2021, Tang et al., 2021, Worpenberg et al., 2021, Zhao et al., 2021, Zykova et al., 2021, Akintayo et al., 2020, Angelova et al., 2020, Arzate-Mejía et al., 2020, Bagci et al., 2020, Bajpai et al., 2020, Barata and Dick, 2020, Basu et al., 2020, Bhargava et al., 2020, Brent and Rajan, 2020, Brown and Evans, 2020, Carnesecchi et al., 2020, Cho et al., 2020, Dold et al., 2020, Duffraisse et al., 2020, Fendl et al., 2020, Funk et al., 2020, González-Méndez et al., 2020, Han et al., 2020, Kiss et al., 2020, Königsmann et al., 2020, Leismann et al., 2020, Levitsky et al., 2020, Liu and Hyeon, 2020, Luhur et al., 2020, Luo et al., 2020, McGough et al., 2020, Moore et al., 2020, Mugat et al., 2020, Muliyil et al., 2020, Nam and Cho, 2020, Olarte et al., 2020, Platenkamp et al., 2020, Ramat et al., 2020, Robles-Murguia et al., 2020, Seif et al., 2020, Sorge et al., 2020, Trivedi et al., 2020, Vandehoef et al., 2020, Vuong et al., 2020, Waghmare et al., 2020, Weber et al., 2020, Wei et al., 2020, Woolley et al., 2020, Xia et al., 2020, Yu and Zallen, 2020, Zhang et al., 2020, Baskar et al., 2019, Bucio-Mendez et al., 2019, Chaplot et al., 2019, Domsch et al., 2019, Drees et al., 2019, Fattouh et al., 2019, Ghosh et al., 2019, Gil-Ranedo et al., 2019, Gultekin and Steller, 2019, Guo et al., 2019, Han et al., 2019, He et al., 2019, Hunter et al., 2019, Imai et al., 2019, Kanca et al., 2019, Katzemich et al., 2019, Kim and Choi, 2019, Kim et al., 2019, Kreutzberger et al., 2019, Lewin et al., 2019, Li et al., 2019, Luhur et al., 2019, Mathre et al., 2019, Meschi et al., 2019, Moreno et al., 2019, Nunes de Almeida et al., 2019, O'Connell et al., 2019, Palazzo et al., 2019, Paré et al., 2019, Petri et al., 2019, Rass et al., 2019, Reynolds et al., 2019, Sharma et al., 2019, Skouloudaki et al., 2019, Skouloudaki et al., 2019, Valoskova et al., 2019, Vijayakumar et al., 2019, von Stockum et al., 2019, Wang et al., 2019, Won et al., 2019, Xu et al., 2019, Zirin et al., 2019, Allen and Seo, 2018, Anding et al., 2018, Baena-Lopez et al., 2018, Bakthavachalu et al., 2018, Balakrishnan et al., 2018, Basso et al., 2018, Baumann et al., 2018, Becker et al., 2018, Billmann et al., 2018, Brown et al., 2018, Courgeon et al., 2018, Cusumano et al., 2018, Duan et al., 2018, Giordano et al., 2018, Ji et al., 2018, Kistler et al., 2018, Knuckles et al., 2018, Kogenaru and Isalan, 2018, Kullmann and Krahn, 2018, Li et al., 2018, Lin et al., 2018, Lin et al., 2018, Lin et al., 2018, Lloyd et al., 2018, Ma and Mayr, 2018, Ma et al., 2018, Mallik et al., 2018, Manieu et al., 2018, Mazzotta et al., 2018, Mohr et al., 2018, Panda et al., 2018, Poon et al., 2018, Reichardt et al., 2018, Ripp et al., 2018, Rosin et al., 2018, Szabo et al., 2018, Tacchelly-Benites et al., 2018, Tang et al., 2018, Wang et al., 2018, Wang et al., 2018, Yatsenko and Shcherbata, 2018, Zouaz et al., 2018, Amarnath et al., 2017, Arenas et al., 2017, Berson et al., 2017, Cherbas et al., 2017.4.19, Epstein et al., 2017, Ewen-Campen et al., 2017, Housden et al., 2017, Hsia et al., 2017, Jeong et al., 2017, Jeske et al., 2017, Kamalesh et al., 2017, Kaur et al., 2017, Li et al., 2017, Lin et al., 2017, Lin et al., 2017, Lippert et al., 2017, Liu et al., 2017, Mallik et al., 2017, Moraru et al., 2017, Myachina et al., 2017, Paik et al., 2017, Palazzo et al., 2017, Perez-Mockus et al., 2017, Pirone et al., 2017, Rajan et al., 2017, Sanfilippo et al., 2017, Song et al., 2017, Spannl et al., 2017, Yeh et al., 2017, Zheng et al., 2017, Ammeux et al., 2016, Bairoch, 2016, Bertolin et al., 2016, Boone et al., 2016, Brinkmann et al., 2016, Chavez et al., 2016, Flegel et al., 2016, Girdler et al., 2016, Guo et al., 2016, Hemalatha et al., 2016, Hurd et al., 2016, Kasture et al., 2016, Lence et al., 2016, Miettinen and Björklund, 2016, Sen and Cox, 2016, Senaratne et al., 2016, Sen et al., 2016, Trost et al., 2016, Truscott et al., 2016, Vianna et al., 2016, Vinayagam et al., 2016, Wang et al., 2016, Wang et al., 2016, Wang et al., 2016, Wang et al., 2016, Zhang et al., 2016, Gaspar et al., 2015, González-Morales et al., 2015, Gordon et al., 2015, Hebbar et al., 2015, Kumichel et al., 2015, Mabashi-Asazuma et al., 2015, Neuhaus-Follini and Bashaw, 2015, Parchure et al., 2015, Ramdas et al., 2015, Ramírez et al., 2015, Stoiber et al., 2015, Verboon et al., 2015, Wu et al., 2015, Zobel et al., 2015, Hwang et al., 2014, Lee et al., 2014, Maier et al., 2014, Qing et al., 2014, Fleming et al., 2013, Manning et al., 2013, Zhang et al., 2013, Colombani et al., 2012, Lloyd et al., 2012, Moin and Urban, 2012, Abell et al., 2011, Cherbas et al., 2011, Tang et al., 2011, Vodovar et al., 2011, Lachkar et al., 2010, Lee et al., 2009, Maurer et al., 2009, Nahvi et al., 2009, Wilkinson et al., 2009, Belenkaya et al., 2008, Cherbas, 2008.11.12, Kaplan et al., 2008, Leibfried et al., 2008, Rencus-Lazar et al., 2008, Wu et al., 2008, Bilic et al., 2007, Franklin-Dumont et al., 2007, Kim et al., 2007, Kondylis et al., 2007, Peretz et al., 2007, Ramadan et al., 2007, Ayoob et al., 2006, Bartscherer et al., 2006, Ma et al., 2006, Rives et al., 2006, Srikanth et al., 2006, Yang and Bashaw, 2006, Bogdan et al., 2005, Gesellchen et al., 2005, Heeger et al., 2005, Sommer et al., 2005, Baeg et al., 2004, Bogdan et al., 2004, Zeidler et al., 2004, Yanagawa et al., 1998)
      S2R+ (DRSC)
      (Han et al., 2022)
      S2R+
      (Ding et al., 2021, Bartscherer et al., 2006, Yi, 2006)
      S2r
      (Zhang et al., 2009)
      S2r+
      (Ripp et al., 2018)
      SL2
      (Cherbas, 2008.11.12)
      SR+
      (Toledano-Katchalski et al., 2007)
      Schneider's 2
      (Cherbas, 2008.11.12)
      Schneider's line 2
      (Cherbas, 2008.11.12)
      Name Synonyms
      Secondary FlyBase IDs
        References (726)