In the presence of continuous Scer\FLP1, P{neoFRT}18A is >75% lethal and 100% of the escapers have severe eye and head defects. The lethality and developmental abnormalities are observed with both male and female flies carrying an P{neoFRT}18A chromosome, so the defects are not due to inter-chromosomal recombination. The P{neoFRT}18A chromosome may carry an aberration (perhaps another FRT site near the one at 18A) and that this has been transmitted to recombinant chromosomes carrying P{neoFRT}18A. I further suspect that recombination between the two FRT sites deletes essential genes, leading to cell lethality and head defects. Wing disc development is disrupted in flies carrying P{neoFRT}18A and Scer\GAL4ap-md544, a continuous source of Scer\FLP1 in the wing. Thus P{neoFRT}18A probably is cell lethal in the presence of continuous Scer\FLP1.
In the presence of continuous Scer\FLP1, P{neoFRT}18A is >75% lethal and 100% of the escapers have severe eye and head defects. The lethality and developmental abnormalities are observed with both male and female flies carrying an P{neoFRT}18A chromosome, so the defects are not due to inter-chromosomal recombination. The P{neoFRT}18A chromosome may carry an aberration (perhaps another FRT site near the one at 18A) and that this has been transmitted to recombinant chromosomes carrying P{neoFRT}18A. I further suspect that recombination between the two FRT sites deletes essential genes, leading to cell lethality and head defects. Wing disc development is disrupted in flies carrying P{neoFRT}18A and Scer\GAL4ap-md544, a continuous source of Scer\FLP1 in the wing. Thus P{neoFRT}18A probably is cell lethal in the presence of continuous Scer\FLP1.
Used to generate mosaics mediated by FLP recombinase; P{neoFRT} insertion at proximal location in chromosome arm.