FlyBase Insertion Report: Dmel\P{PZ}hh[rJ413]

General Information

Symbol

Dmel\P{PZ}hh^rJ413

Species

D. melanogaster

Name

FlyBase ID

FBti0002944

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)rJ413

Affected gene(s)

CG31457, Ecol\lacZ, hh

Viability / fertility

Associated allele(s)

Ecol\lacZ^hh-rJ413, hh^rJ413

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 94E1 )

Sequence Location

3R:23,141,947..23,141,947 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:23,141,947..23,141,947 [-]

(Levis, 2008.1.9)

Orientation

(Levis, 2008.1.9)

Cytological location (computed by FlyBase)

94E1 (inferred by FlyBase from sequence location)

Cytological location (reported)

94E1-94E4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

May correspond to 2 insertions or a single insertion associated with a deletion: based on the GenBank records, the two flanks of this line map 14.6 kb apart. The 3' flank maps at 3R: 18953010, which is in CG31457, and the 5' flank maps at 3R: 18967669, which is at the 5' end of hh.

(Levis, 2008.1.9)

Genomic location previously reported as 3R:18952917..18952917 [+].

(BDGP Project Members, 1994-1999)

Sequence Data

Flanking sequence

AQ026358

(FlyBase, 1992-)

AQ034132

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

CG31457

(FlyBase, 1996-)

Ecol\lacZ

(Gorfinkiel et al., 2003, Moreno and Morata, 1999, Casares et al., 1997, Huang and Kunes, 1996, Guillen et al., 1995, Pan and Rubin, 1995, Heberlein et al., 1993)

(Guillen et al., 1995)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^hh-rJ413

Phenotypic Class Phenotype Manifest in Detailed Description

hh^rJ413

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter