FlyBase Insertion Report: Dmel\P{PZ}Ten-m[05309]

General Information

Symbol

Dmel\P{PZ}Ten-m⁰⁵³⁰⁹

Species

D. melanogaster

Name

FlyBase ID

FBti0003563

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)05309

Affected gene(s)

Ecol\lacZ, Ten-m

Viability / fertility

viable

Associated allele(s)

Ecol\lacZ^Ten-m-05309, Ten-m⁰⁵³⁰⁹

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 79D4-79E3 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

79D4-79E3 (near gene of known cytology)

Cytological location (reported)

79E (in situ hybridization reported)

(FlyBase, 1992-)

79E1--79E2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AF008224

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Vosshall, 1998.8.10, Braun et al., 1997, Levine et al., 1997, Levine et al., 1997, Baumgartner et al., 1994, Levine et al., 1994)

Ten-m

(Jin et al., 2020, Zhang et al., 2018, Zheng et al., 2011, Levine et al., 1997, Baumgartner et al., 1994, BDGP Project Members, 1994-1999, Levine et al., 1994)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Ten-m-05309

Phenotypic Class Phenotype Manifest in Detailed Description

Ten-m⁰⁵³⁰⁹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval midgut | restricted

(Meister and Braun, 1995.10)

adepithelial cell of eye-antennal disc

(Levine et al., 1997)

eye disc morphogenetic furrow

(Levine et al., 1997)

clypeo-labral disc | uniform

(Levine et al., 1997)

labial disc | uniform

(Levine et al., 1997)

wing pouch | restricted

(Levine et al., 1997)

presumptive 3rd axillary sclerite

(Levine et al., 1997)

presumptive 4th axillary sclerite

(Levine et al., 1997)

sensory mother cell of wing disc

(Levine et al., 1997)

proximal costal vein | precursor

(Levine et al., 1997)

wing hinge primordium

(Levine et al., 1997)

presumptive axillary cord

(Levine et al., 1997)

leg disc | restricted

(Levine et al., 1997)

humeral disc | uniform

(Levine et al., 1997)

prothoracic trochanter | precursor

(Levine et al., 1997)

sensory mother cell of leg disc

(Levine et al., 1997)

embryonic/larval optic lobe | restricted

(Levine et al., 1997)

larval ventral nerve cord | restricted

(Levine et al., 1997)

midgut imaginal island

(Levine et al., 1997)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

antenna

(Levine et al., 1997)

maxillary palp

(Levine et al., 1997)

alula

(Levine et al., 1997)

axillary cord

(Levine et al., 1997)

proximal costal vein

(Levine et al., 1997)

1st axillary sclerite

(Levine et al., 1997)

3rd axillary sclerite

(Levine et al., 1997)

4th axillary sclerite

(Levine et al., 1997)

wing vein L3

(Levine et al., 1997)

campaniform sensillum of proximal dorsal radius

(Levine et al., 1997)

prealar apophysis

(Levine et al., 1997)

dorsal pedicellar campaniform sensillum

(Levine et al., 1997)

ventral pedicellar campaniform sensillum

(Levine et al., 1997)

chordotonal organ of scabellum

(Levine et al., 1997)

dorsal scabellar campaniform sensillum

(Levine et al., 1997)

ventral scabellar campaniform sensillum

(Levine et al., 1997)

capitellum

(Levine et al., 1997)

humerus

(Levine et al., 1997)

Additional Information

Statement

Reference

The expression pattern of EcollacZ^Ten-m-05309 is essentially identical to that of EcollacZ^Ten-m-00844.

(Levine et al., 1997)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)05309

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11657

P{PZ}TAF1C-like^05309b; P{PZ}Ten-m⁰⁵³⁰⁹ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

Originally isolated from line carrying multiple insertions.

(BDGP Project Members, 1994-1999)

Evidence suggests that the "l(3)05309" line contains 2 P{PZ} insertions; one at 57E (P{PZ}05309b) corresponding to the site of the flanking sequence from this line (AQ073328), and one at 79E (P{PZ}Ten-m⁰⁵³⁰⁹) which causes a mutation in Ten-m.

(FlyBase, 1992-)

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P1657

(Meister and Braun, 1995.10)

P[PZ]Ten-m05309

(Jin et al., 2020)

P{PZ}Penh22

(FlyBase, 1992-)

P{PZ}Ten-m⁰⁵³⁰⁹

(BDGP Project Members, 1994-1999, Levine et al., 1994, FlyBase, 1992-)

P{PZ}l(3)05309⁰⁵³⁰⁹

(FlyBase, 1992-)

P{ry^+t7.2=PZ}Ten-m⁰⁵³⁰⁹

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000206

References (22)

Report Sections

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