FlyBase Insertion Report: Dmel\P{PZ}spen[03350]

General Information

Symbol

Dmel\P{PZ}spen⁰³³⁵⁰

Species

D. melanogaster

Name

FlyBase ID

FBti0003918

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)03350

Affected gene(s)

Ecol\lacZ, spen

Viability / fertility

Associated allele(s)

Ecol\lacZ^spen-03350, spen⁰³³⁵⁰

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 21B4 )

Sequence Location

2L:161,799..161,799 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:161,799..161,799 [+]

Orientation

Cytological location (computed by FlyBase)

21B4 (inferred by FlyBase from sequence location)

Cytological location (reported)

21B4-21B6 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00736

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Vosshall, 1998.8.10, Montell et al., 1992)

spen

(Girard et al., 2020, Montell et al., 1992)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^spen-03350

Phenotypic Class Phenotype Manifest in Detailed Description

spen⁰³³⁵⁰

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval foregut

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

embryonic/larval brain

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

ring gland

(Meister and Braun, 1995.10)

embryonic/larval salivary gland

(Meister and Braun, 1995.10)

adult stage

adult brain

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In adult, expression throughout brain.

(Vosshall, 1998.8.10)

In wandering third instar larva, high levels of expression in imaginal discs (which discs not specified), throughout gut, and in salivary glands; slightly lower levels of expression in Malpighian tubules and fat body; weak expression in brain and ring gland.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)03350

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11295

P{PZ}spen⁰³³⁵⁰ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1295

(Meister and Braun, 1995.10)

P{PZ}l(2)03350⁰³³⁵⁰

(FlyBase, 1992-, Montell et al., 1992)

P{PZ}spen⁰³³⁵⁰

(Girard et al., 2020, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)03350⁰³³⁵⁰

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000316

References (14)

Report Sections

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