FB2024_03 , released June 25, 2024
Insertion: Dmel\P{PZ}Cyt-b501857
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General Information
Symbol
Dmel\P{PZ}Cyt-b501857
Species
D. melanogaster
Name
FlyBase ID
FBti0005202
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

l(2)01857

Affected gene(s)
Cyt-b5, Ecol\lacZ
Viability / fertility
Associated allele(s)
Cyt-b501857, Ecol\lacZCyt-b5-01857
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 43D3 )
Sequence Location
2R:7,534,235..7,534,235 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:7,534,235..7,534,235 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
43D3 (inferred by FlyBase from sequence location)
Cytological location (reported)
43E4-43E5 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location

The insertion site is reported to be at +792 relative to the Cyt-b5 translation start site.

(Kleinhesselink et al., 2011)
Sequence Data
Flanking sequence
AQ025591
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ring gland
(Jackle and Janning, 1998.8.10)
embryonic labral segment
(Jackle and Janning, 1998.8.10)
embryonic maxillary segment | restricted
(Jackle and Janning, 1998.8.10)
embryonic stage 3 -- 4
organism | anterior
(Harvie et al., 1998)
embryonic stage 10
ring gland | precursor
(Harvie et al., 1998)
larval stage
ring gland
(Harvie et al., 1998)
third instar larval stage
eye disc
(Harvie et al., 1998)
adult stage
maxillary palp
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference

In early embryos, expression at anterior pole. In mid-late embryos, expression in ring gland precursor cells. Expressed in ring gland during all larval stages; only other significant larval expression is in the the eye disc of third instar larva.

(Harvie et al., 1998)

In embryo, expression in maxillary segment and in ring gland not certain.

(Jackle and Janning, 1998.8.10)
 
Marker for
ring gland
(Harvie et al., 1998)
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)01857

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2R:3046048-3046049 confirmed by FlyBase alignment of dbGSS accession AQ025591 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
01857
(Kleinhesselink et al., 2011)
P{PZ}Cyt-b501857
(FlyBase, 2005, FlyBase, 1992-)
P{PZ}l(2)0185701857
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0185701857
(FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
  • FBti0000281
References (14)