Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
In wandering third instar larva, no detectable β-gal staining.
l(2)s4831
Deficiency complementation analysis is consistent with the lethality of the line being associated with the insertion. The insertion maps within the HnRNP-K transcription unit. However, since the insertion lies within a gene-rich region, the lethality can not yet be conclusively mapped to a single gene from these results. Thus l(2)s4831s4831 (representing the lethality of the chromosome) has been kept as a separate allele in FlyBase for now.
Location 2R:16103255-16103256 confirmed by FlyBase alignment of dbGSS accession AQ026148 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.