FB2024_03 , released June 25, 2024
Insertion: Dmel\P{lacW}vrik05901
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General Information
Symbol
Dmel\P{lacW}vrik05901
Species
D. melanogaster
Name
FlyBase ID
FBti0006274
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(2)k05901

Affected gene(s)
Ecol\lacZ, vri
Viability / fertility
Associated allele(s)
Ecol\lacZvri-k05901, vrik05901
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
2L ( 25D5 )
Sequence Location
2L:5,306,915..5,306,915 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:5,306,915..5,306,915 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
25D5 (inferred by FlyBase from sequence location)
Cytological location (reported)
25D4-25D5 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AQ034151
(FlyBase, 1992-)
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Insertion may affect gene
Ecol\lacZ
(George and Terracol, 1997)
vri
(George and Terracol, 1997)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 10 -- 17
embryonic/larval foregut
(George and Terracol, 1997)
embryonic stage 13
embryonic/larval posterior spiracle
(George and Terracol, 1997)
embryonic stage 13 -- 16
anal pad
(George and Terracol, 1997)
third instar larval stage
embryonic/larval brain
(George and Terracol, 1997)
embryonic/larval digestive system
(George and Terracol, 1997)
imaginal disc
(George and Terracol, 1997)
adult stage & oogenesis stage S8
border follicle cell
(George and Terracol, 1997)
Additional Information
Statement
Reference

Enhancer trap staining is observed in embryos in the foregut, posterior spiracles and anal pads. In ovaries, staining is observed in border cells and in columnar follicular epithelial cells. In third instar larvae, staining is observed in the gut, brain, and imaginal discs.

(George and Terracol, 1997)
 
Marker for
Reflects expression of
vri
(George and Terracol, 1997)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)k05901

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2L:5306915-5306916 confirmed by FlyBase alignment of dbGSS accession AQ034151 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{lacW}vrik05901
(FlyBase, 2005, George and Terracol, 1997, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (13)