Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
EcollacZtkv-k16713 expression is stronger in the upper part of the air sac primordium than the lower part.
EcollacZtkv-k16713 is expressed along the anterior and posterior sides of the wing pouch; expression is excluded from a wide vertical band perpendicular to the D/V boundary in the middle of the wing pouch. EcollacZtkv-k16713 is expressed throughout the presumptive capitellum (haltere pouch).
Larval expression complementary to that of bi in the wing disc.
l(2)k16713
Location 2L:5237595-5237596 confirmed by FlyBase alignment of dbGSS accession AQ026112 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).