Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Comment: at the ends
In late third instar larvae, reporter expression is observed in three regions of the notum. One contains and lies distal to the posterior notopleural bristle (pNP) and lies posterior to the anterior notopleural bristle (aNP). One lies distal to the trichoid sensillum (tri1). Expression is also observed along the AP boundary of the prospective wing blade. In pupal wings beginning at 25 APF, expression is observed around the crossveins. Expression is also seen at the ends of the longitudinal veins. Reporter expression is also observed along the AP boundary of the leg disc and antennal disc and posterior to the morphogenetic furrow in the eye disc.
l(2)k03511
No longer maintained in the Berkeley P stock collection.
Line not well characterized
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).