FlyBase Insertion Report: Dmel\P{lacZ}loco[3-109]

General Information

Symbol

Dmel\P{lacZ}loco^3-109

Species

D. melanogaster

Name

FlyBase ID

FBti0009142

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacZ}

LINE ID

Affected gene(s)

Ecol\lacZ, loco

Viability / fertility

Associated allele(s)

Ecol\lacZ^loco-3-109, loco^3-109

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 94B7 )

Sequence Location

3R:22,626,614..22,626,967 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:22,626,614..22,626,967 [-]

(Granderath et al., 1999)

Orientation

(Granderath et al., 1999)

Cytological location (computed by FlyBase)

94B7 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

The P{lacZ}loco^3-109 insertion site maps between an EcoRI site and the loco-RC transcription start and is reported to be within 1 bp of P{lacZ-un1}loco^rC56.

(Granderath et al., 1999)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{lacZ}

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Tayler et al., 2004, Granderath et al., 1999, Huang and Kunes, 1998, Park et al., 1997, Perez and Steller, 1996, Ito et al., 1995, Martin et al., 1995, Sawamoto et al., 1995, Campbell et al., 1994, Xiong et al., 1994, Winberg et al., 1992, Klambt and Goodman, 1991)

loco

(Granderath et al., 1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^loco-3-109

Phenotypic Class Phenotype Manifest in Detailed Description

loco^3-109

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

lateral ectoderm | dorsal

(Granderath et al., 1999)

embryonic stage 12 -- 17

glial cell

(Granderath et al., 1999)

embryonic stage 16

embryonic longitudinal glial cell

(Ito et al., 1995)

segmental nerve root glial cell

(Ito et al., 1995)

larval channel glial cell

(Ito et al., 1995)

subperineurial glial cell

(Ito et al., 1995)

intersegmental nerve root glial cell

(Ito et al., 1995)

embryonic/larval cell body glial cell

(Ito et al., 1995)

first instar larval stage

glial cell of embryonic/larval central nervous system

(Ito et al., 1995)

embryonic/larval central nervous system | restricted

(Ito et al., 1995)

Additional Information

Statement

Reference

Enhancer trap expression is detected at embryonic stage 12 in cells which appear to be progeny of the lateral glioblast. Those cells migrate medially and divide. By the end of embryogenesis, enhancer trap expression is observed in most glial cells except the midline glia. Expression is also seen in the dorsal leading edge cells of the lateral ectoderm.

(Granderath et al., 1999)

Marker for

Reflects expression of

loco

(Granderath et al., 1999)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

The P{lacZ}3-109 insertion site maps between an EcoRI site and the loco-RC transcription start and is reported to be within 1 bp of P{lacZ-un1}loco^rC56. It is in a positive orientation with respect to loco gene transcription.

(Granderath et al., 1999)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{lacZ}3109

(FlyBase, 1992-, Klambt and Goodman, 1991)

P{lacZ}loco^3-109

(Granderath et al., 1999, FlyBase, 1992-)

loco:LacZ

(Tayler et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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