Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.
A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.
Insertion reported at coordinate 147222 on AE003809(.1).
GFP-Zasp52 is an early marker for myofibril assembly and a suitable tool for live imaging studies of myofibril assembly. It clears from areas next to myotendinous junctions and forms clusters which sort out into Z-discs and coalesce to form the final Z-discs.
