Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.
A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.
The insertion maps to the 5' end of the first intron of lost.
Reporter expression is observed in nurse cells and in the oocyte, where it becomes
enriched early in oogenesis. During mid-oogenesis, GFPLost is concentrated at the oocyte anterior margin and is also weakly detected at the posterior cortex. In later stage embyros, it is enriched in the cortex both anteriorly and posteriorly. In early embryos, it is concentrated at the posterior pole and is incorporated into pole cells.
The orientation of the insertion was not reported.