FlyBase Insertion Report: Dmel\P{GawB}NP0225

General Information

Symbol

Dmel\P{GawB}NP0225

Species

D. melanogaster

Name

FlyBase ID

FBti0058535

Feature type

transposable_element_insertion_site

Also Known As

NP225-GAL4, NP225, GAL4-NP225, NP225Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP0225

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 51A4 )

Sequence Location

2R:14,479,031..14,479,031 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:14,479,031..14,479,031 [-]

(Potter and Luo, 2010)

Orientation

(Potter and Luo, 2010)

Cytological location (computed by FlyBase)

51A4 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Approximate location; corresponds to estimated site of insertion of mdg3 element in the P{GawB}NP0225 parental strain.

(Potter and Luo, 2010)

Repetitive region: within mdg3 element (not found at this location in the sequenced stain).

(Potter and Luo, 2010)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Awasaki et al., 2011, Michels et al., 2011, Pitman et al., 2011, Pauls et al., 2010, Potter and Luo, 2010, Tamura et al., 2010, Donlea et al., 2009, Grosjean et al., 2008, Tanaka et al., 2008, Wang et al., 2006, Masuda-Nakagawa et al., 2005, Tanaka et al., 2004)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP0225

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

adult antennal lobe projection neuron | subset

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus A1

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus A2

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus A3

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus A4

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus D4

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus D1

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus D5

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus I2

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus I4

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus L1

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus L2

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus L6

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus L8

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus M2

(Masuda-Nakagawa et al., 2005)

larval MB calyx glomerulus V5

(Masuda-Nakagawa et al., 2005)

adult stage

adult antennal lobe projection neuron | subset

(Potter and Luo, 2010, Tanaka et al., 2004)

adult antennal lobe projection neuron

(Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

adult medial antennal lobe tract

(Tanaka et al., 2008)

adult lateral horn | restricted

(Potter and Luo, 2010)

adult antennal lobe glomerulus | subset

(Tanaka et al., 2004)

unilateral antennal lobe projection neuron | subset

(Tanaka et al., 2012)

adult antennal lobe projection neuron D adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DA2 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DL1 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DL4 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DL5 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DM1 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DM2 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DM3 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DM4 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DM5 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DM6 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA2 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA3 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA4 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA6 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VM2 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VM3 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA7l adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VC1 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VC2 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VM7v adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DA1 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DA3 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DA4l adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DC3 adPN

(Israel et al., 2022, Tanaka et al., 2012)

adult antennal lobe projection neuron DL3 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA1d adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA1v adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DC2 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VA7m lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VL2a adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VL2p lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VM1 lPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron VM4 adPN

(Tanaka et al., 2012)

adult antennal lobe projection neuron DP1m adPN

(Tanaka et al., 2012)

adult uniglomerular antennal lobe projection neuron | subset

(Tanaka et al., 2012)

medial antennal lobe tract projection neuron

(Flybrain Neuron Database et al., 2018.6.28)

mushroom body pedunculus-vertical lobe arborizing neuron 1

(Flybrain Neuron Database et al., 2018.6.28)

uniglomerular antennal lobe projection neuron | subset

(Israel et al., 2022)

adult neuron | subset of adult subesophageal zone

(Israel et al., 2022)

adult neuron | subset of adult ventral nerve cord

(Israel et al., 2022)

Additional Information

Statement

Reference

Drives expression in a subset of larval mushroom body calyx glomeruli and of uniglomerular antennal lobe projection neurons.

(FlyBase Curators, 2017)

ScerGAL4^NP0225 drives expression in around 68 uniglomerular antennal lobe projection neurons. The cell bodies of around 31 of these neurons are positioned in the anterior-dorsal part of the antennal lobe. Around 29 cell bodies are found in the lateral part and around 9 are positioned ventrally. These neurons project to a large number of antennal lobe glomeruli including VM7d and VP4.

(Tanaka et al., 2012)

Expression is observed in 67-73 projection neurons in 35 of 43 antennal glomeruli.

(Tanaka et al., 2004)

Marker for

adult antennal lobe projection neuron

(Tanaka et al., 2008)

Reflects expression of

Reporter construct used in assay

nSyb^UAS.cEa.GFP

(Masuda-Nakagawa et al., 2005)

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2012, Potter and Luo, 2010, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

112095

w^*; P{GawB}NP0225 / CyO, P{UAS-lacZ.UW14}UW14

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

GAL4-NP225

(Okada et al., 2009, Wang et al., 2006)

NP0225

(Flybrain Neuron Database et al., 2018.6.28)

NP0225-GAL4

(Min et al., 2013)

NP225

(Israel et al., 2022, Naganos et al., 2022, Aso et al., 2014, Scheunemann et al., 2012, Tanaka et al., 2012, Awasaki et al., 2011, Pitman et al., 2011, Pauls et al., 2010, Tamura et al., 2010, Donlea et al., 2009, Tanaka et al., 2008, Masuda-Nakagawa et al., 2005, Tanaka et al., 2004)

NP225-GAL4

(Mohamed et al., 2023, Sabandal et al., 2022, Wong et al., 2021, Sabandal et al., 2020, Bakthavachalu et al., 2018, Aranda et al., 2017, Lin et al., 2014, Potter and Luo, 2010)

NP225-Gal4

(Zhou et al., 2019, Badel et al., 2016, Sudhakaran et al., 2014, Michels et al., 2011)

NP225-gal4

(Gai et al., 2016)

NP225Gal4

(McCann et al., 2011, Grosjean et al., 2008)

P{GawB}NP0225

(FlyBase, 1992-)

P{GawB}NP225

(Tanaka et al., 2004, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (34)

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