Expression is seen within all mushroom body lobes in pharate adults, but fades in non-alpha/beta lobes. Eighteen larval abdominal ganglion cells are seen sending projections towards the midline, but this number reduces to 12 in early pupae, and 6 (as three pairs) in pharate adults. Expression is also seen in a small subset of adult peripheral sensory neurons that innervate the female reproductive structures. Expression in the larval R8 cell fades beyond 48hrs after pupal formation (APF). Expression in the eye, mushroom body, subesophageal ganglion and ventral nerve cord is still present post-eclosion. Labelled cells are first seen in the ventral nerve cord 48hrs after egg laying; in the subesophageal ganglion earlier than 8hrs after egg laying; in the mushroom body neurons throughout larval and pupal stages; and in cells of the eye within 48hrs after egg laying.