FlyBase Insertion Report: Dmel\P{PTT-un}vkg[G00454]

General Information

Symbol

Dmel\P{PTT-un}vkg^G00454

Species

D. melanogaster

Name

FlyBase ID

FBti0153267

Feature type

transposable_element_insertion_site

Also Known As

Vkg-GFP, Vkg::GFP, Viking-GFP, vkg^G454.GFP, G454, Vkg.GFP, vkg^G454-GFP

Description

Inserted Element

P{PTT-un}

LINE ID

Affected gene(s)

Avic\GFP, vkg

Viability / fertility

Associated allele(s)

Avic\GFP^vkg-G00454, vkg^G00454

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 25C1 )

Sequence Location

2L:5,024,627..5,024,627 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PTT-GA,GB,GC}.FT

Description

Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.

(Buszczak et al., 2007)

A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w^+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.

(Morin et al., 2001)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:5,024,627..5,024,627 [-]

(Morin et al., 2001)

Orientation

(Morin et al., 2001)

Cytological location (computed by FlyBase)

25C1 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Insertion reported at coordinate 84156 on AE003608.1.

(Morin et al., 2001)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PTT-un}

(Morin et al., 2001)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Avic\GFP^vkg-G00454

Phenotypic Class Phenotype Manifest in Detailed Description

vkg^G00454

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval lymph gland | restricted

• extracellular matrix

(Jung et al., 2005)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

embryonic/larval cardial cell

(Drechsler et al., 2013)

Additional Information

Statement

Reference

Vkg::GFP protein localizes to the basal side of cardial cells.

(Drechsler et al., 2013)

AvicGFP^vkg-G00454 is localized to an intricate extracellular matrix network forming chamber-like structures within the cortical zone of the lymph gland.

(Jung et al., 2005)

Marker for

basement membrane

(Yasunaga et al., 2010)

embryonic/larval cardial cell

(Drechsler et al., 2013)

Reflects expression of

vkg

(Yasunaga et al., 2010, Jung et al., 2005, Morin et al., 2001)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

98343

y¹ w^*; P{PTT-un}vkg^G00454

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (36)

Reported As

Symbol Synonym

COL4A2::GFP

(Kelemen-Valkony et al., 2012)

ColIVα2-GFP-trap

(Matsubayashi et al., 2020)

Collagen IV-GFP

(Lodge et al., 2021)

Collagen::GFP

(Anllo et al., 2019)

G00454

(Guha et al., 2009)

G00454II

(Lovegrove et al., 2019)

G454

(Jung et al., 2005, Pagliarini and Xu, 2003, Morin et al., 2001)

P^PTT-un1vkgG454

(Csordás et al., 2020)

P{PTT-un1}vkg^G00454

P{PTT-un1}vkg^G454

(FlyBase, 1992-)

P{PTT-un}vkg^G00454

VKG::GFP

(Yasunaga et al., 2010)

Viking-GFP

(Rackley et al., 2021, Lovegrove et al., 2019, Kelemen-Valkony et al., 2012, Srivastava et al., 2007)

Viking::GFP

(Petley-Ragan et al., 2016)

Viking:GFP

(Stork et al., 2008)

Vkg-GFP

(Liu et al., 2022, Lovegrove et al., 2019, Itoh et al., 2018, Romani et al., 2016, Van De Bor et al., 2015, Pastor-Pareja and Xu, 2011, Olofsson and Page, 2005)

Vkg.GFP

(Yang et al., 2021, Sun et al., 2019, Liu et al., 2017)

Vkg::GFP

(Csordás et al., 2020, Yoshinari et al., 2020, Zappia et al., 2020, Drechsler et al., 2013)

Vkg^G454-GFP

(Zheng et al., 2020)

Vkg^G454.GFP

(Liu et al., 2022)

col4a2::GFP

(Kelemen-Valkony et al., 2012)

collagen-IV-GFP

(Khan et al., 2013)

viking-GFP

(Bunt et al., 2011, Widmann and Dahmann, 2009)

viking^G454

(Pastor-Pareja and Xu, 2011)

vkg-GFP

(Peng et al., 2022, Chen et al., 2019, Proag et al., 2019, Khan et al., 2013, Tang et al., 2008)

vkg-GFP (G00454)

(Arefin et al., 2014)

vkg-GFP^G00454

(Zappia et al., 2020)

vkg-GFP[G0454]

(Proag et al., 2019)

vkg-gfp

(Weavers and Skaer, 2013, Bunt et al., 2010)

vkg::GFP

(Koper et al., 2012)

vkg::GFP-454

(Drechsler et al., 2013)

vkg^G00454-GFP-trap

(Hauling et al., 2014)

vkg^G454

(Pastor-Pareja and Xu, 2011)

vkg^G454-GFP

(Sun et al., 2021)

vkg^G454.GFP

(Yang et al., 2021, Sun et al., 2019, Dai et al., 2018, Liu et al., 2017)

vkgG454-GFP

(Zhou et al., 2023)

Name Synonyms

Secondary FlyBase IDs

FBti0129818
FBti0027874

References (47)

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