FB2024_03 , released June 25, 2024
Insertion: Dmel\Mi{PT-GFSTF.1}Ten-mMI02844-GFSTF.1
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General Information
Symbol
Dmel\Mi{PT-GFSTF.1}Ten-mMI02844-GFSTF.1
Species
D. melanogaster
Name
FlyBase ID
FBti0178469
Feature type
transposable_element_insertion_site
Description
Inserted Element
Mi{PT-GFSTF.1}
LINE ID

MI02844-GFSTF.1

Affected gene(s)
Ten-m
Viability / fertility
Associated allele(s)
Ten-mMI02844-GFSTF.1
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3L ( 79E2 )
Sequence Location
3L:22,385,699..22,385,699 [-]
Target / Docking site
Mi{MIC}Ten-mMI02844
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_Mi{PT-GFSTF.0,1,2}.GDP
Description

A set of transgenic insertion stocks containing a protein trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The protein trap cassette contains a EGFP-FlAsH-StrepII-TEV-3xFlag ("GFSTF") tag that is flanked on either side with a 4X(GlyGlySer) flexible linker; the mini-yellow marker is no longer present. Each insertion line contains one of three versions of the cassette (Mi{PT-GFSTF.0}, Mi{PT-GFSTF.1} or Mi{PT-GFSTF.2}), depending on the reading frame required to generate an in-frame fusion protein with the gene in which the insertion is located. This system also allows UAS/GAL4-based knockdown strategies targeting GFP-sequence containing mRNA (RNAi against GFP) or GFP fusion protein (deGradFP).

(Nagarkar-Jaiswal et al., 2015)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:22,385,699..22,385,699 [-]
(Gene Disruption Project members, 2015-)
Orientation

-

(Gene Disruption Project members, 2015-)
Cytological location (computed by FlyBase)
79E2 (inferred by FlyBase from sequence location)
Cytological location (reported)
79E2_r6 (reported as inferred from sequence location)
(Gene Disruption Project members, 2015-)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Mi{PT-GFSTF.1}
(Gene Disruption Project members, 2015-)
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Insertion may affect gene
Ten-m
(Kamiyama et al., 2021, Zhang et al., 2018, Gene Disruption Project members, 2015-)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

MI02844-GFSTF.1

(Gene Disruption Project members, 2015-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Linkouts
GDP-RMCE
  • RM00154
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
EGFP-tagged Ten-m
(Kamiyama et al., 2021)
Mimic-Ten-m::GFP
(Zhang et al., 2018)
Mi{PT-GFSTF.1}Ten-mMI02844-GFSTF.1
(Kamiyama et al., 2021, Gene Disruption Project members, 2015-)
Name Synonyms
Secondary FlyBase IDs
    References (4)