FB2024_03 , released June 25, 2024
Recombinant Construct: P{hsneo}
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General Information
Symbol
P{hsneo}
FlyBase ID
FBtp0000078
Feature type
transgenic_transposable_element
Component Allele(s)
Size
5.075
Expression Data
Associated insertion(s)
154 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Tn\neoRHsp70Bb.PS
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
X01803
(bases 1-5073 )
Segments and Size
Total Size

5.075

Left end

P5\'

(Steller and Pirrotta, 1985)
Right end

P3\'

(Steller and Pirrotta, 1985)
 
Segments
Num
Orientation
Symbol
1
+
pUChsneo_P5'(P5',H3)
2
-
PUC8mcs
3
+
pUChsneo_P3'(R1,P3')
Reference
(Steller and Pirrotta, 1985)
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{hsneo}
(Steller and Pirrotta, 1985)
descendant(s)
Comments

construct_comment: Conflicts in sequence data: discrepancies in Hsp70 region; FlyBase-compiled sequence based upon available sequence data for Hsp70Bb.

(Steller and Pirrotta, 1985)

report comment: Conflicts in sequence data: additional 2 bases at P{6.1} deletion site probably in error (see FBrf0038996); bases 4850-4851 in (sequence entry X01803 | 5705 | 06 Jul 1989) omitted in FlyBase-compiled sequence.

(Steller and Pirrotta, 1985)

construct_comment: Restriction sites useful for plasmid rescue: EcoRI, SmaI, BamHI, HindII, SalI.Series of constructs derived from pP{hsneo} differ in cloning sites (cloning sites listed in construct reports).

(Steller and Pirrotta, 1985)
Stocks (17)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{hsneo}
(FlyBase, 1996-, Steller, 1996.10.18, FlyBase, 1992-, Cooley et al., 1988)
Secondary FlyBase IDs
    References (12)