FB2024_03 , released June 25, 2024
Recombinant Construct: P{CaSpeR-lacZ}
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General Information
Symbol
P{CaSpeR-lacZ}
FlyBase ID
FBtp0000165
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZhs43
Size
9.595
Expression Data
lacZ reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZhs43
Transgenic product class
Component
Use(s)
Encoded product / tool
Ecol\lacZ
Description

Construct: Ecol\lacZ is transcribed from a minimal promoter consisting of the Hsp70 TATA box, deleted at -43, and leader sequences.

(Thummel and Pirrotta, 1991)
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
X81643
(bases )
Segments and Size
Total Size

9.595

Left end

P5\'

(Thummel and Pirrotta, 1991)
Right end

P3\'

(Thummel and Pirrotta, 1991)
 
Segments
Num
Orientation
Symbol
1
+
CaSpeR_P5'(P5',Sp)
2
+
pW8mcs-St
3
+
CaSpeR-lacZ_P3'(r,P3')
Reference
(Thummel and Pirrotta, 1991)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference

β-gal activity observed in transient assay (using larval fat body) without heat shock.

(McKenzie and Brennan, 1996)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{CaSpeR-lacZ}
(Thummel and Pirrotta, 1991)
descendant(s)
Comments

report comment: Compiled sequence initiates at different position and is in opposite orientation relative to sequence entry listed.CAUTION: GenBank entry X81643 | 551429 | 29 Sep 1994 appears to be in error; relative to other P{CaSpeR} constructs, 60bp missing after coordinate 8505. See also report for component segments w+mC4.1(Sp) : sequence discrepancies.

(Thummel and Pirrotta, 1991)

construct_comment: Allows testing of regulatory sequences in front of lacZ reporter gene.

(Thummel and Pirrotta, 1991)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
CaSpeR_hs43_lacZ
(Thummel and Pirrotta, 1991)
P{CaSpeR-lacZ}
(FlyBase, 1996-, Pirrotta, 1994.9.14)
Secondary FlyBase IDs
    References (7)