P\TΔ2-3 lacks a tissue-specific intron that normally limits transposase activity to the germline.
The last intron, which is responsible for the limitation of transposase activity to the germline, has been deleted. P\TΔ2-3 therefore produces transposase in both the somatic and germ cells.
Precise deletion of the intron sequences at the ORF2 and ORF3 junction.
10.25
P5\'
P3\'
construct_comment: Allows expression of P transposase in somatic as well as germ cells.The most famous member of a series of constructs used to characterize the role of RNA splicing in the regulation of P transposase.