FlyBase Recombinant Construct Report: P{hsH\T-2}

General Information

Symbol

P{hsH\T-2}

FlyBase ID

FBtp0000488

Feature type

transgenic_transposable_element

Component Allele(s)

hobo\T^HSH2

Also Known As

HSH2

Size

11.255

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

ry^+t7.2

Component allele hobo\T^HSH2

Transgenic product class

Component

Use(s)

Encoded product / tool

hobo\T

Description

Construct: Fusion of Hsp70 sequences from -245 to +207, relative to the start of transcription, to position 147 of hobo, 40bp downstream of the hobo TATA consensus.

(Calvi and Gelbart, 1994)

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

11.255

Left end

P5\'

(Calvi and Gelbart, 1994)

Right end

P3\'

(Calvi and Gelbart, 1994)

Segments

Num

Orientation

Symbol

Reference

(Calvi and Gelbart, 1994)

Phenotypic Data

Component Allele

Phenotypic Data

hobo\T^HSH2

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{hsH\T-2}

(Calvi and Gelbart, 1994)

descendant(s)

Comments

report comment: Ig = unspecified immunoglobulin sequences.

(Ish-Horowicz and Pinchin, 1987)

construct_comment: Heat-induced expression of H\T results in activity in both soma and germline. Included as non-attributed correction.

(Calvi and Gelbart, 1994)

construct_comment: One of a series of heat-shock-hairy constructs cloned into neoR vectors.

(Ish-Horowicz and Pinchin, 1987)

sequence comment: CAUTION: May be additional fragment between Hsp70Bb sequences and ry⁺ sequences.

(Calvi and Gelbart, 1994)

construct_comment: Description as \'source immobile\' applies to stability when exposed to H-element transposase; subject to transposition when exposed to P-element transposase.

(Calvi and Gelbart, 1994)

mapping_comment: CAUTION: May be additional fragment between Hsp70Bb_P450(Xb,Xm) and ry⁺7.2(H3).

(Calvi and Gelbart, 1994)

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

6602

y¹ w^67c23; In(2LR)Gla, wg^Gla-1/CyO, P{hsH\T-2}CyO-1

Kyoto

108847

y¹ w^67c23; In(2LR)Gla, wg^Gla-1/CyO, P{hsH\T-2}CyO-1

External Crossreferences and Linkouts ( 0 )