FlyBase Recombinant Construct Report: P{GAL4-twi.2xPE}

General Information

Symbol

P{GAL4-twi.2xPE}

FlyBase ID

FBtp0001322

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^twi.2PE

Also Known As

2xPE-GAL4, 2xPE-twi-GAL4, twist-Gal4, twi.2xPE-GAL4, twi::Gal4, twi-GAL4, twist2xPE-Gal4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

3 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Scer\GAL4^twi.2PE

Transgenic product class

Component

Use(s)

Regulatory region(s)

twi

Encoded product / tool

GAL4

binary expression system - driver

Description

Construct: BamHI and XbaI were used to remove Ecol\lacZ and replace it with Scer\GAL4^wB.

(Schubiger, 1997.3.14)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^twi.2PE

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

mesoderm

(Mendoza-García et al., 2017)

embryonic stage 5

organism | ventral

(Schubiger, 1997.3.14)

Additional Information

Statement

Reference

Drives mesodermal expression

(FlyBase Curators, 2017)

In embryos at cellular blastoderm, expressed at high levels in the ventralmost 12-14 cells, with sharp boundaries at lateral borders.

(Schubiger, 1997.3.14)

Marker for

Reflects expression of

twi

(Mendoza-García et al., 2017, Schubiger, 1997.3.14)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{GAL4-twi.2xPE}

(Schubiger, 1997.3.14)

descendant(s)

Comments

report comment: Bam HI and Xba I were used to remove β-gal and replace it with GAL4.

(Schubiger, 1997.3.14)

report comment: 2xPE element comparable to that in P{twi-lacZ.2PE}.

(Schubiger, 1997.3.14)

Stocks (9)

Stocks containing insertion(s) of this recombinant construct

Bloomington

2517

y¹ w^*; P{GAL4-twi.2xPE}2

58804

y¹ w^*; P{GAL4-twi.2xPE}3

84311

w^*; P{GAL4-twi.2xPE}1; P{UAS-GC3Ai}3

84290

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-CC3Ai}3

84332

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-VC3Ai}3

25707

P{UAS-Dcr-2.D}1, w¹¹¹⁸; P{GAL4-twi.2xPE}1

67053

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-3xFLAG.dCas9.VPR}attP2

67084

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-Cas9.P2}attP2/TM6B, Tb¹

Kyoto

107376

y¹ w^*; P{GAL4-twi.2xPE}2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

2 X PE twi-Gal4

(Lee et al., 2003)

2xE-twi-GAL4

(Ismat et al., 2010)

2xPE-GAL4

(Mendoza-García et al., 2017, Schaub and Frasch, 2013, Schaub et al., 2012)

2xPE-Gal4

(Ákos et al., 2021, Wolfstetter et al., 2017, Boukhatmi et al., 2014)

2xPE-twi-GAL4

(Jammrath et al., 2020, Schwarz et al., 2018, Reim and Frasch, 2005, Reim et al., 2005, Wang et al., 2005)

GAL4-twi.2xPE

(Garcia et al., 2011)

P{GAL4-twi.2xPE}

(Wolfstetter et al., 2017, Zappia and Frolov, 2016, Günesdogan et al., 2014, FlyBase, 1996-)

Twist-Gal4

(Loren et al., 2003)

twi-GAL4

(Domsch et al., 2021, Macabenta and Stathopoulos, 2019)

twi-Gal4

(Frasch et al., 2023)

twi.2xPE-GAL4

(Park et al., 2011, Lo et al., 2002)

twi.2xPE-Gal4

(Mendoza-Garcia et al., 2021)

twi::Gal4

(Yadav et al., 2021, Klingseisen et al., 2009, van Impel et al., 2009)

twi::Gal4(2x)

(van Impel et al., 2009)

twi_l-GAL4

(Sun and Stathopoulos, 2018)

twiPE

(Morris et al., 2006)

twist-2XPE-gal4

(Zimmermann et al., 2006)

twist-Gal4

(Deshpande et al., 2014, Serpe and O'Connor, 2006, Petryk et al., 2003, Marques et al., 2002)

twist.2xPE-Gal4

(Uçkun et al., 2021)

twist2xPE-Gal4

(Hugosson et al., 2014, Popichenko et al., 2013)

twistGal4

(Popichenko et al., 2013)

Secondary FlyBase IDs

References (38)

Report Sections

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