FB2024_03 , released June 25, 2024
Recombinant Construct: P{CecA1.A10-lacZ}
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General Information
Symbol
P{CecA1.A10-lacZ}
FlyBase ID
FBtp0004803
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZCecA1.A10
Also Known As
cec-lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mW.hs
Component allele Ecol\lacZCecA1.A10
Transgenic product class
Component
Use(s)
Regulatory region(s)
CecA1_pA10
Encoded product / tool
lacZ
Description

882bp HindIII-HphI fragment of upstream untranslated CecA1 sequences from position -760 to +62 drives expression of lacZ.

(Engstrom et al., 1993)

lacZ expression is regulated by upstream untranslated region of CecA1 from -760bp to +62bp. CecA1 sequence includes the R1, κΒ-like and R2 motifs.

(Petersen et al., 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14 -- 16
yolk
(Tingvall et al., 2001)
embryonic stage 17
embryonic epidermis
(Tingvall et al., 2001)
larval stage
embryonic/larval fat body
(Engstrom et al., 1993)
adult stage | male
ejaculatory duct
(Junell et al., 2010)
adult stage
adult anterior midgut
(Dantoft et al., 2013)
Additional Information
Statement
Reference

Strong, tissue-specific expression is observed in the male ejaculatory duct.

(Junell et al., 2010)

In embryos, no expression observed in fat body or hemocytes. Embryonic expression assayed after injection with LPS (3-4 hours earlier).

(Tingvall et al., 2001)

In larvae, LPS-induced expression in all fat body tissue; high degree of tissue-specificity. Induction is achieved by injection of LPS (bacterial liposaccharide) into the body cavity; mimics response to bacterial infection. No expression observed in control animals not exposed to LPS.

(Engstrom et al., 1993)

LPS-inducible expression also observed in blood cell line mbn-2.

(Engstrom et al., 1993)
 
Marker for
Reflects expression of
CecA1
(Dantoft et al., 2013, Junell et al., 2010)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Symbol Synonym
A10 CecA1-lacZ
(Tingvall et al., 2001)
CecA1-LacZ(pA10)
(Lindberg et al., 2018)
CecA1-lacZ
(Junell et al., 2010)
CecA1-lacZ A10
(Tingvall et al., 2001)
CecA1-lacZ pA10
(Dantoft et al., 2013)
P{CecA1.A10-lacZ}
(FlyBase, 1996-)
cec-lacZ
(Basset et al., 2000, Uv et al., 2000)
pA10
(Petersen et al., 1999)
pA10 Cecropin A1-lacZ
(Hedengren-Olcott et al., 2004)
Secondary FlyBase IDs
    References (14)