FB2024_03 , released June 25, 2024
Recombinant Construct: P{7.1-ci}
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General Information
Symbol
P{7.1-ci}
FlyBase ID
FBtp0004874
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZci.7.1
Also Known As
ci-lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\lacZci.7.1
Transgenic product class
Component
Use(s)
Regulatory region(s)
ci_7.1
Encoded product / tool
lacZ
Description

7.1kb fragment of ci upstream and transcribed sequences (first 5 codons) is fused to codon 8 of lacZ. Comparison of the ci fragment to genomic sequence reveals a 135bp deletion 1.2kb upstream from the start of transcription.

(Schwartz et al., 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ventral midline of embryo
(Schwartz et al., 1995)
embryonic stage 5

Comment: expressed in two broad stripes

(Schwartz et al., 1995)
extended germ band stage
organism | segmentally repeated
(Schwartz et al., 1995)
embryonic stage 10 -- 11
ventral midline of embryo
(Schwartz et al., 1995)
larval stage
wing disc anterior compartment
(Schwartz et al., 1995)
Additional Information
Statement
Reference

The 7.1 kb ci upstream region drives EcollacZ expression in a pattern approximating wild type ci transcript expression. The protein is detected in the cellular blastoderm stage, and persists through germ band retraction in a segmentally repeated pattern. In the cellular blastoderm stage, two segments of EcollacZ expression are detected, rather than the three segments seen in wild type ci expression. Expression is also detected along the ventral midline. In imaginal discs, reporter expression is restricted to the anterior compartment.

(Schwartz et al., 1995)
 
Marker for
Reflects expression of
ci
(Schwartz et al., 1995)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
7.1-ci
(Ahmad and Baker, 2002)
P{7.1-ci}
(FlyBase, 1996-)
ci-7.1-LacZ
(Chanas et al., 2004)
ci-lacZ
(Ahmad and Baker, 2002, Randsholt et al., 2000, Zhang and Kalderon, 2000)
ci-lacZ. cy000801.
(Randsholt et al., 2000)
Secondary FlyBase IDs
    References (8)